Fig. 5

Coding gene RNA expression. A Heatmap showing an unsupervised hierarchical clustering (Euclidean distances) of the differences in immune gene expressions between before and after (week 3) treatment with atezolizumab. The clustering was made on log2-transformed TPM values of 536 immune-related genes extracted from the LM22 dataset after pre-processing, in patients for whom both baseline and week 3 RNAseq data were available. Pink refers to higher expression at baseline (reduction of expression at week 3) and green refers to lower expression at baseline (increased expression at week 3) B-C. Differential expression analyses of top-differentiated genes among the 536 immune-related genes when comparing only elite responders (> 1 year) with the others (B) and patients who rapidly progressed with the others (C). CCL19, BHLHE41, CXCL9, MS4A6A, GZMB, DGKA, BIRC3, PDK1, MACF1 and SLAMF1 expressions are commonly found of good prognosis