Fig. 2

PaSSS-based therapy surpasses clinically prescribed Taxol and is patient-specific. A Barcode representing basal PaSSS of BR98 tumor, namely the set of active unbalanced processes. BR98 harbors one process, process 1. B Zoom-in image of the unbalanced process 1 active in BR98. The complete list of proteins that participate in the process 1 can be found in Table S1. Black circles denote upregulated proteins due to the process (as calculated using a product \({G}_{i\alpha }{\lambda }_{\alpha }\left(k\right)\), Supplementary Methods), gray circles represent downregulated proteins in the same process. Functional connections are according to STRING database. C BR98 tissues were orthotopically transplanted into NSG mice at the age of 6–7 weeks. Once tumors reach 60–80 mm³ volume, PaSSS predicted therapy (Erlotinib) and paclitaxel were used to assess the response of the tumors to these therapeutic strategies. At day 37 the treatment was stopped and tumor growth continued to be monitored. D Barcode representing basal PaSSS of PDX11. E Zoom-in image of the unbalanced processes active in PDX11 as well as the predicted drugs targeting the central proteins in each process. The complete list of proteins that participate in each of the processes can be found in Table S3. Black circles denote upregulated proteins due to the process (as calculated using a product \({G}_{i\alpha }{\lambda }_{\alpha }\left(k\right)\)), gray circles represent downregulated proteins in the same process. Functional connections are according to STRING database. F PDX11 tissues were orthotopically transplanted as described in C. The experimental groups included control (vehicle) group, erlotinib monotherapy group, AG-1024 monotherapy group (anti-IGFR), Taxol chemotherapy, Erlotinib (Er) + AG-1024 (PaSSS therapy) and Er + Trametinib (Tr) (combination predicted to be less effective). Treatments were carried out for 36 days. On day 37 the treatments were stopped and tumors were grown untreated