Fig. 3

circPSD3 inhibits the migration and invasion of HCC cells in vitro. (A and B) The relative expression levels of circPSD3 and linear PSD3 in HCC-LM9 and SK-Hep-1 cells stably transfected with circPSD3 overexpression or control lentivirus were measured by qRT-PCR. (C and D) The relative expression levels of circPSD3 and linear PSD3 in HCC-LM9 and SK-Hep-1 cells stably transfected with circPSD3 shRNA (sh-circPSD3-1/2) or scramble shRNA (sh-NC) lentivirus was measured by qRT-PCR. (E and F) Representative images and quantification of the wound healing assays in circPSD3-overexpressing, circPSD3 knockdown, and control HCC cells. scale bar = 100 μm. (G and H) Representative images and quantification of the Transwell migration and invasion assays in circPSD3-overexpressing, circPSD3 knockdown, and control HCC cells. scale bar = 100 μm. (I and J) Representative images and quantification of the 3D spheroid-based invasion assay in circPSD3-overexpressing and control HCC cells. scale bar = 100 μm. Data are shown as the mean ± SD. Statistical analyses were performed using unpaired Student’s t-tests (NS, no statistical significance; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). circPSD3, circRNA pleckstrin and Sect. 7 domain containing 3; HCC, hepatocellular carcinoma; qRT-PCR, quantitative real-time PCR