Fig. 1

Study Overview. The overall strategy used to develop HCC-specific MS-HRM assay is shown. The first panel displays the number of normal (N) and HCC (T) methylome data from CGRC and TCGA cohorts obtained using Infinium microarray (top). The scatter plot of the Random Forest classification importance rank for markers in two cohorts is shown, with the common markers labeled with a red dotted circle (bottom). The second panel depicts the random forest models used to select HCC-specific markers and the number of samples used to build the model (top), as well as a t-SNE plot that distinguishes HCC from other cell types when clustered with the selected methylation markers (bottom). The third panel shows the CpG sites differentially methylated and their melting curves from MS-HRM analysis (top). The formulas for the methylation score and the sum of methylation score are shown below. The last panel shows the number of blood samples used for the HCC liquid biopsy test and the test results in bar graph along with the sensitivity of the assays shown