Fig. 2

The construction of a cfDNA methylation-based CRC screening model and validation by a prospective high-risk population cohort. A Hierarchically clustered heatmap demonstrating the cfDNA methylation increment of the selected markers in CRC patients of training set. B ROC plots the of cfDNA methylation-based model and commonly used protein biomarkers for predicting CRC in training set. Random forest was used for training the cfDNA methylation-based model. The ROC curve of the methylation model was plotted based on out-of-bag prediction results. C Hierarchically clustered heatmap showing the methylation alternations of the selected markers in CRC patients of test set. D ROC curves of the methylation model and protein biomarkers for CRC detection in test set. E ROC curves for CRC detection by the methylation model and protein biomarkers in the prospective cohort. F Overall sensitivity and specificity. G Sensitivity of the CRC detection model for early and late-stage CRC. H Positive prediction values for cfDNA methylation-based model and protein markers