Fig. 2

New insights on the transport of nanoparticles (NPs) through endothelial cells using intravital microscopy (IVM). Panel (A) shows NP transport through gaps between adjacent endothelial cells in dynamic vascular bursts, while panel (B) shows NP transport across the endothelial cell layer via transcytosis. Panel (C) presents representative images of eruptions occurring near and without leukocyte cells, respectively, using 70 nm Doxil particles and a BxPC3-GFP dorsal skinfold model. Panel (D) demonstrates colocalization of NPs with endothelial cells to form hotspots along the vessel lining in MMTVPyMT and 4T1 tumor models using 50 nm AuNPs conjugated with Alexa Fluor 647. The scale bars for all panels are provided, and insets are included where appropriate. Reprint from [47] with a permission from Elsevier