Fig. 1

Spatial visualisation of virtual biopsies. We used our recently published organ-wide spatial transcriptomic data [9] to construct virtual biopsy models (2 tumour biopsies and 1 benign biopsy) that mimic conventional biopsy placement and core size. Visualisation of histology and tissue status (GG: Gleason grade group; PIN: prostatic intraepithelial neoplasia) and tumour clones from each tumour biopsy. Spatial visualisation of gene expression (ANPEP, ANO7, TPM2 and REPS2) in each tumour biopsy. Violin plots representing gene expression according to histological status. TRUS = transrectal ultrasound guided prostate biospy. LATP = local anaesthetic transperineal prostate biospy. ST = spatial transcriptomics. GG = Gleason grade group. PIN = prostatic intra-epithelial neoplasia. 18G = 18 gauge core biopsy needle