Fig. 3

EYA4 regulates proliferation and migration in cells. A EYA4 knockdowns in HeLa cells (mean ± SEM; 3 biological replicates). β-Actin was used as the loading control. Western blot was repeated at least three times. Quantification was done on the represented immunoblot. B Representative growth curves of HeLa control and EYA4 depleted cells. Proliferation was measured by live imaging. Data represent the mean relative confluency ± SEM of three independent experiments. C Representative images of an in vitro wound-healing assay monitored by live imaging and the corresponding quantification of the migration area after wounding are shown. Data represent the mean wound confluency ± SEM of three independent experiments. D Annexin V-labeled apoptotic HeLa cells were measured by live imaging. Data represent the mean total integrated intensity ± SEM of three independent experiments. For all panels * P ≤ 0.05, *** P ≤ 0.001, **** P ≤ 0.0001