Fig. 2

EYA4 promotes breast cancer development through its serine/threonine phosphatase domain. Analysis of distant metastasis after mouse lateral tail-vein injections. A and G Metastasis was monitored at the indicated time points by whole animal bioluminescence imaging (BLI). Quantification of the BLI signal is plotted (mean ± SEM; n = 3 for A, and n = 5 for G). B and H Representative images of surgically removed livers (scale bar 5 mm) are shown. White arrows indicate liver metastatic foci. C-E and I-K Analysis of liver metastatic foci. C and I Hematoxylin and eosin staining of livers dissected 4–5 weeks post tail-vein injection. Low magnification (scale bar 5 mm), mid magnification (scale bar 500 μm), and high magnification views (scale bar 50 μm). Black boxes indicate the areas shown at higher magnification. Quantification (D and J; median; n = 3 for D, and n = 5 for J) and area (E and K; mean ± SEM) of liver metastatic foci are plotted. F Liver metastatic site with central necrosis (➞), accumulation of inflammatory cells (➨) and blood vessel congestion (➔), shown in left panel; and fibrosis (➞), shown in right panel. Scale bar 50 μm. For all panels * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001