Fig. 7

LINC00922 altered H3K27cr occupation via SIRT3. (A) Heatmaps and signal density plots centered on transcriptional start sites of genes targeted by SIRT3. (B) Immunofluorescence images depicting the location of SIRT3 (green) and H3K27cr (red) in HCT116 cells. Scale bar, 5 μm. (C) Immunoblotting analysis of H3K27cr level in HCT116 cells transfected with SIRT3 plasmid for 48 h. (D) RIP-qPCR analysis of LINC00922-SIRT3 interaction in HCT116 cells with or without SIRT3 transient overexpression for 48 h (n = 3). (E) RNA-FISH and immunofluorescence staining of LINC00922 RNA (red) and SIRT3 protein (green) in HCT116 cells. Scale bar, 5 μm. (F) RIP-qPCR analysis of LINC00922-H3K27cr interaction in HCT116 cells. (G) ChIP-qPCR analysis of SIRT3 enrichment on the EST1 promoter in HCT116 cells with LINC00922 stable knockdown or transient overexpression for 48 h (n = 3). H Immunoblotting analysis of ETS1 expression in HCT116 cells co-transfected with LINC00922 and SIRT3 plasmids for 48 h. The numbers below western bands indicate the ratio of protein versus GAPDH or H3 in experimental groups to that in control group, analyzed using Image J2. Data are represented as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, unpaired, two-tailed, Student’s t-test