Fig. 5

LINC00922 promoted invasion and migration via ETS1. (A-B) Representative images of Transwell (A) and wound healing assay (B) depicting motility of cells following ETS1 overexpression for 48 h (left panel). Scale bar, 200 μm. The number of cells and percent of wound closure were counted in 3 random fields (right panel). (C) Immunoblotting analysis of E-Cad, Vimentin, Snail1, ETS1, and GAPDH levels in CRC cells subjected to ETS1 transient overexpression for 48 h. (D-E) Representative images of Transwell assay (D) and wound healing assay (E) depicting motility in HCT116 cells co-transfected with LINC00922 plasmid and si-ETS1 for 48 h (left panel). Scale bar, 200 μm. The number of cells and percent of wound closure were counted in 3 random fields (right panel). (F) Immunoblotting analysis of Vimentin, Snail1, ETS1, and GAPDH levels in CRC cells co-transfected with LINC00922 plasmid and si-ETS1 for 48 h. The numbers below western bands indicate the ratio of protein versus GAPDH in experimental groups to that in control group, analyzed using Image J2. Data are represented as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, unpaired, two-tailed, Student’s t-test