Fig. 4

LINC00922 promoted invasion and migration via H3K27cr mediated CAMs in epithelial cells. (A) Immunoblotting analysis of E-Cad, Vimentin, and Snail1 expression in LINC00922 stable knockdown HCT116 cells treated with 10 mM NaCr for 48 h. The numbers below western bands indicate the ratio of protein versus GAPDH in experimental groups to that in control group, analyzed using Image J2. (B) Transwell assay images demonstrating the level of invasion and migration in LINC00922 stable knockdown HCT116 cells treated with 10 mM NaCr for 48 h (left panel). Scale bar, 200 μm. Cells were counted in 3 random fields (right panel). Data are represented as means ± SD, *P < 0.05, unpaired, two-tailed, Student’s t-test. (C) H3K27cr peaks distribution in HCT116 cells transfected with the LINC00922 or control plasmid for 48 h. (D) KEGG analysis of enrichment pathways of genes annotated by 21,253 different H3K27cr peaks in HCT116 cells with or without transient LINC00922 overexpression for 48 h. (E) UMAP plotting single cell transcriptome profiles of CRC tissues (GSE196964). The color represents different cell types (left panel) or tissue types (right panel). (F) Enrichment of 653 genes and other histone crotonylation-related gene sets across various cell types of colon tissues (left panel) and CRC tissues (right panel). The significance was calculated using GSEA. Dot indicates P < 0.05, and plus symbol indicates P ≥ 0.05. The red point represents gene set positively expressed in the corresponding cell type, and the blue point represents the opposite. The size of dot represents the negative base 10 logarithm of p-value. (G) UMAP plotting single cell transcriptome profile of CRC tissues (GSE225857). The color represents different cell types (left panel) or tissue types (right panel). (H) Heatmap showing the enrichment of pathways annotated by 653 genes in epithelial cells derived from primary CRC and LM tissues. The NES was calculated using GSEA. The red represents pathway positively enriched in the epithelial cells, and the blue represents the opposite. LM: liver metastasis