Fig. 3

LINC00922 promoted CRC metastasis. (A-B) Representative images of transwell assay showing the invasion and migration levels of CRC cells after instantaneous knockdown (A) or overexpression (B) of LINC00922 for 48 h. Scale bar, 200 μm. Cells were counted in 3 random fields (middle panel). (C) Illustrations of LINC00922 stable knockdown HCT116 cell-derived lung metastases. The arrow indicates the tumor nodule. (D) Comparison of lung metastasis nodules between the sh-NC and sh-LINC00922 groups. (E-G) Immunoblotting analysis of E-Cad, Vimentin, Snail1, and GAPDH levels in CRC cells with LINC00922 transient knockdown (E), transient overexpression (F), or stable knockdown (G). The numbers below western bands indicate the ratio of protein versus GAPDH in experimental groups to that in control group, analyzed using Image J2. Data are represented as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, unpaired, two-tailed, Student’s t-test