Fig. 2
Association of LINC00922 with CRC metastasis and poor prognosis. (A) Enrichment of genes with promoter occupied by histone crotonalytion across various dysregulated lncRNAs. The significance was calculated using GSEA. Dot indicates P < 0.05, and plus symbol indicates P ≥ 0.05. The red point represents gene set positively expressed with the corresponding lncRNA, and the blue point represents the opposite. The size of point represents the negative base 10 logarithm of p-value. NES: normalized enrichment score. (B) Immunoblotting analysis of H3K27cr level in HCT116 cells transfected with LINC00922 plasmid for 48 h. The numbers below H3K27cr indicate the ratio of H3K27cr versus H3 in experimental groups to that in control group, analyzed using Image J2. (C) Kaplan-Meier survival curve analysis of the association of LINC00922 expression with overall survival (OS, n = 326) in CRC samples from the TCGA cohort. (D) The Mann-Whitney U analysis of LINC00922 expression level between normal and CRC tissues from the TCGA database. ****P < 0.0001. E-F. Mann-Whitney U analysis of LINC00922 expression in CRC samples from the TCGA cohort with or without distant metastasis (E), or with or without lymph node invasion (F). **P < 0.01, ****P < 0.0001. G. Meta-analysis of the relationship between LINC00922 expression and distant metastasis in CRC samples (n = 972) from the GEO database