Fig. 1

Clinical results showing H3K27cr was associated with CRC metastasis. (A) Representative immunohistochemistry images depicting H3K27cr level across different types of CRC tissues. Scale bar, 500 μm. (B-D) Mann-Whitney U (B-C) and one-way ANOVA (D) analysis of H3K27cr level across different types of CRC tissues. The 45 cases of tumor samples in Fig. 1C contained 10 cases of distant metastatic colorectal cancer tissues, 16 cases of primary colorectal cancer tissues, 11 cases of colorectal adenoma tissues, and 8 cases positive lymph node tissues. (E) Immunoblotting analysis of H3K27cr level in HCT116 cells treated with 10 mM NaCr for 48 h. The numbers below H3K27cr indicate the ratio of H3K27cr versus H3 in experimental groups to that in control group, analyzed using Image J2. (F-G) Transwell assay images demonstrating the levels of invasion and migration in HCT116 (F) and LoVo cells (G) treated with 10 mM NaCr for 48 h (left panel). Scale bar, 200 μm. Cells were counted in 3 random fields (right panel). Data are represented as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, unpaired, two-tailed, Student’s t-test