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Fig. 6 | Molecular Cancer

Fig. 6

From: circEIF3I facilitates the recruitment of SMAD3 to early endosomes to promote TGF-β signalling pathway-mediated activation of MMPs in pancreatic cancer

Fig. 6

circEIF3I facilitates recruitment of SMAD3 to TGFβRI on early endosomes. A and B Western blot analysis of indicated proteins in Flag-TGFβRI immunoprecipitated complexes or in HA-SMAD3 immunoprecipitated complexes from lysates of PANC-1 and BxPC-3 with circEIF3I KD (5 ng/ml TGF-β, 15 min). C Representative IF images identified the colocalization of SMAD3 (white arrows) and TGFβRI in PANC-1 cells transfected with sh-ctrl or sh-circEIF3I-2 (5 ng/ml TGF-β, 15 min). The red indicated SMAD3, the green indicated the TGFβRI, the blue (DAPI) indicated the nucleus (Scale bar = 10 μm). D and E Western blot showed the p-SMAD3 expression (5 ng/ml TGF-β, 15 min) in cells transfected with sh-ctrl or sh-circEIF3I-2, with or without the endocytosis blocked by potassium depletion or Dyngo-4a (30 μM). F Representative IF images identified the colocalization of SMAD3 and EEA1 in PANC-1 cells transfected with sh-ctrl or sh-circEIF3I-2 (5 ng/ml TGF-β, 15 min). The red indicated SMAD3, the green indicated the EEA1, the blue (DAPI) indicated the nucleus (Scale bar = 10 μm). G The fluorescence intensity of regions of interest (ROI) was quantified along with the indicated white dashed using ImageJ software

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Molecular Cancer

ISSN: 1476-4598