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Fig. 4 | Molecular Cancer

Fig. 4

From: circEIF3I facilitates the recruitment of SMAD3 to early endosomes to promote TGF-β signalling pathway-mediated activation of MMPs in pancreatic cancer

Fig. 4

circEIF3I promotes invasion and metastasis by increasing MMPs expression and activity. A The heatmap showing the expression profiles of genes related to collagen degradation pathway. B qRT-PCR analyses of relative mRNA expression of MMP2, MMP9 and MMP14 in PANC-1 and BxPC-3 cells transfected with sh-ctrl or sh-circEIF3I-2. C and D The expression of MMP2, MMP9 (10 ng/ml PMA) and MMP14 was determined by western blot in PANC-1 and BxPC-3 cells with circEIF3I KD (C) and overexpression (D). E Gelatine zymography for conditioned media (10 ng/ml PMA, 24 h) of PANC-1 and BxPC-3 cells with circEIF3I KD and overexpression. F and G Representative images of immunohistochemistry analysis to detect the expression of MMP2, MMP9 and MMP14 in orthotopic tumours (Scale bar = 50 μm). Data are shown as the mean ± SD of three replicates; *P < 0.05; **P < 0.01; ns, not significant

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Molecular Cancer

ISSN: 1476-4598