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Fig. 2 | Molecular Cancer

Fig. 2

From: circEIF3I facilitates the recruitment of SMAD3 to early endosomes to promote TGF-β signalling pathway-mediated activation of MMPs in pancreatic cancer

Fig. 2

Identification and characteristics of circEIF3I. A Schematic diagram of circEIF3I generated from the Exon 6 and Exon 7 of EIF3I gene. B Sanger sequencing of head-to-tail splice junction in circEIF3I. C The agarose gel electrophoresis was performed using the divergent primers and convergent primers to identify the existence of circEIF3I. D qRT-PCR assay to determine circEIF3I, EIF3I mRNA, and GAPDH mRNA levels in samples after RNase R treatment. E qRT-PCR showed the stability of circEIF3I and EIF3I mRNA in pancreatic cancer cell treated with 5 μg/ml actinomycin D (Act D) for 24 h. F and G Representative FISH images identified the subcellular distribution of circEIF3I in pancreatic cancer cell (F) and PDAC tissues (G) using circEIF3I specific probe. The green (FAM-labelled probe) indicated the circEIF3I, while the blue (DAPI) indicated the nucleus (Scale bar = 10 μm). Data are shown as the mean ± SD of three replicates; **P < 0.01; ***P < 0.001; ns, not significant

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Molecular Cancer

ISSN: 1476-4598