Fig. 2

circEHD2 promotes proliferation, migration, and invasion of RCC cells. A and B, The knockdown efficiency (A) and overexpression (B) of circEHD2 in RCC cells measured by qRT-PCR. C and D, The proliferation ability of OSRC-2 (C) and 786-O cells (D) with circEHD2 knockdown was measured by CCK-8 assays. E and F, EdU assay revealed that silencing circEHD2 suppressed the proliferation ability of OSRC-2 and 786-O cells. Scale bars: 200 μm. G and H, The colony formation ability of OSRC-2 and 786-O cells was reduced when transfecting with sh-circEHD2s. I and J, The invasion abilities of OSRC-2 and 786-O cells were inhibited by the knockdown of circEHD2 through Transwell assays. Scale bars: 200 μm. K and L, The migration abilities of OSRC-2 and 786-O cells were inhibited by silencing circEHD2 via Transwell assays. Scale bars: 200 μm. Error bars, standard deviation (SD) of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001