Fig. 1

circEHD2 was upregulated in ccRCC tissues and positively associated with poor prognosis. A and B, The cluster heat maps showed the differentially expressed circRNAs in four ccRCC tissues and paired NATs from GSE100186; and the differentially expressed circRNAs in seven RCC tissues and paired NATs from GSE108735. The red and green strips indicate up-regulated and down-regulated circRNAs, respectively. C, The overlapping analysis of upregulated circRNAs in GSE100186 and GSE108735. D, The expression level of circEHD2 in 80 ccRCC tissues and paired normal adjacent tissues (NATs) by qRT-PCR. E and F, The expression level of circEHD2 in a cohort of 80 ccRCC patients stratified by stage and ISUP grade. G and H, Representative images and quantitative analyses for the circEHD2 expression in ccRCC tissues and paired NATs via FISH. Scale bars: 50 μm. I, Representative HE images and ISH images from scramble probe (red: negative control), U6 probe (blue: positive control) and circEHD2 probe in ccRCC tissues and paired NATs. Scale bar: 50 μm. J. The percentages of circEHD2 expression in ccRCC tissues and NATs. K, ROC curve analysis verified that tissue-circEHD2 could discriminate ccRCC from NAT with high accuracy. L and M, Kaplan-Meier curves of Overall survival (OS) and Progression-free survival (PFS) according to the level of tissue-circEHD2 in ccRCC patients (n = 80). The cut-off values were the median expression of circEHD2 in ccRCC tissues. **, P < 0.01; ***, P < 0.001