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Fig. 1 | Molecular Cancer

Fig. 1

From: Spatially resolved, high-dimensional transcriptomics sorts out the evolution of biphasic malignant pleural mesothelioma: new paradigms for immunotherapy

Fig. 1

Morphology guided spatial transcriptomics on B-MPM tissues

(A) Representative H&E staining of B-MPMs included in the training set. Magnification 100X. (B) Experimental design of the spatial transcriptomic analysis. Three types of regions were selected based on cell morphology and expression of Pan-CK marker. pE-AOIs, pS-AOIs corresponded to area of pure epithelioid and sarcomatoid component respectively. Transitional regions (t-AOIs) containing both mixed epithelioid and sarcomatoid cells were further segmented in tE-AOIs and tS-AOIs. Collected AOIs were sequenced and transcriptional profiles analysed. (C) GeoMx DSP scan showing AOIs from a representative B-MPM collected from a surgical biopsy. Large circles indicate segmented transitional AOIs while small circles circle indicate pure AOIs. Insight displays the enlargement of representative transitional areas. (D) Principal component analysis (PCA) distribution of total E-AOIs and S-AOIs, based on differentially expressed genes (adjusted p-value < 0.05). (E) Principal component analysis distribution of the indicated AOIs, based on differentially expressed genes (adjusted p-value < 0.05)

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Molecular Cancer

ISSN: 1476-4598