Fig. 4
From: LINC02159 promotes non-small cell lung cancer progression via ALYREF/YAP1 signaling
ALYREF is upregulated in NSCLC and promotes NSCLC cell proliferation, migration and invasion. A The expression of ALYREF in LUAD tissues (unpaired and paired samples) using data from TGCA. B ROC curve of ALYREF in NSCLC tissues from TCGA database. C Correlation analyses of ALYREF expression and NSCLC patients’ overall survival in the GEPIA database. D TCGA database analysis of the correlation between ALYREF expression and clinical stages. E and F Knockdown efficiency of ALYREF in A549 and PC9 cells was assessed by qRT-PCR and western blot. G Inhibited CCK-8 assays for the growth rate of control and ALYREF knockdown NSCLC cells. H The efficiency of plate colony formation in A549 and PC9 cells after ALYREF knockdown. I and J Transwell migration and Matrigel invasion assays for control and ALYREF knockdown groups (200×). K and L Flow cytometry analyses of cell apoptosis and cell cycle distribution in control and ALYREF knockdown groups. M and N Western blot were performed to determine the effects of ALYREF knockdown on the expression of growth and metastasis-related proteins in A549 and PC9 cells. (*P < 0.05, **P < 0.01, ***P < 0.001, ns: no significance)