Fig. 3
From: LINC02159 promotes non-small cell lung cancer progression via ALYREF/YAP1 signaling
LINC02159 interacts with ALYREF protein and positively correlates with ALYREF in NSCLC. A Schematic diagram of the TRAP experiment. B and C Silver staining and western blot to detect the expression of GST after TRAP. D qRT-PCR was used to measure the expression level of LINC02159 in the product after TRAP. E LINC02159 was verified to bind to ALYREF by western blot. F RIP experiments for the interaction between ALYREF and LINC02159. G FISH and immunofluorescence staining for the co-localization of LINC02159 and ALYREF in NSCLC cells. H and I qRT-PCR and western blot analyses of ALYREF protein and mRNA expression in control and LINC02159-knockdown NSCLC cells. J Immunofluorescence staining of ALYREF in control and LINC02159-knockdown NSCLC cells. K Subcellular fractionation experiment for ALYREF protein in the nucleus and cytoplasm of control and LINC02159 knockdown NSCLC cells. L The expression of ALYREF protein in 20 paired NSCLC tissues and adjacent non-tumor tissues was detected by western blot. (*P < 0.05, **P < 0.01, ns: no significance)