Fig. 2

LINC02159 exerts oncogenic roles in NSCLC in vitro. A NSCLC cell lines A549 and PC9 cells were transfected with LINC02159 siRNA or control siRNA, and the knockdown efficiency was detected by qRT-PCR. B and C The proliferation capacity of A549 and PC9 cells after LINC02159 knockdown was evaluated by the CCK-8 assay (B) and colony-forming assay (C). D The percentage of apoptosis in the control group and LINC02159 knockdown group was analyzed by flow cytometry. E Flow cytometry analyses of cell cycle distribution after knockdown of LINC02159. Data are presented as mean ± SD (n = 3). F The effect of LINC02159 knockdown on the expression of proliferation and apoptosis-related proteins in A549 and PC9 cells. G Transwell migration assay was performed to determine the effects of LINC02159 knockdown on A549 and PC9 cell migration (200×). H The invasion ability of A549 and PC9 cells after LINC02159 knockdown was measured by using Matrigel invasion assay (200×). I Western blot analyses of EMT-related protein expression in control and LINC02159 knockdown groups. J The tumor weight and tumor size in mice of control and LINC02159 knockdown groups. K Sliced mouse tumor tissues were subjected to H&E, Ki-67, and TUNEL staining (scale bars = 100 μm). (*P < 0.05, **P < 0.01, ***P < 0.001, ns: no significance)