Fig. 5
From: The use of RNA-based treatments in the field of cancer immunotherapy
The impact of different intracellular delivery methods on in vitro and in vivo functionality. a In vitro colony-forming assays are used to compare the differentiation potential of electroporated and squeezed human CD34 + HSCs into CFU-GM and BFU-E colonies over a 2-week period. b The viability of mouse T cells after undergoing squeeze and electroporation is displayed. c and d The proportion of CD3 + mouse T cells expressing PD-1 or CD69 activation after squeeze, electroporation, or no treatment (control) is presented over time. e A diagram illustrates the experimental method for evaluating the effects of delivery methods on T cell activation. f and g On day 4, after re-exposure to OVA, CD45.2 + /CD8 + /IFN-γ + T cells were stained intracellularly for IFN-γ. Reprinted from [127] with permission from the Proceedings of the National Academy of Sciences