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Fig. 5 | Molecular Cancer

Fig. 5

From: A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer

Fig. 5

STK38, the interacting protein of CAPG-171aa, promotes the proliferation, migration, and invasion capacity of TNBC. (A) Nine interacting proteins of circCAPG were selected through MS and listed with their corresponding scores. (B) IB of these nine potential interacting proteins of CAPG-171aa through Co-Immunoprecipitation assay with flag antibody in TNBC cells. (C) Western blotting analysis of STK38 in TNBC and its corresponding adjacent normal tissue. (D) Cell proliferation in STK38 overexpressed (OE-STK38) MDA-MB-231 and MDA-MB-468 cells. (E) Colony formation assay in OE-STK38 transfected MDA-MB-231 and MDA-MB-468 cells. (F) Immunofluorescence of GFP in OE-STK38 transfected MDA-MB-231 and MDA-MB-468 cells. (G) Western blotting analysis of CDK4 and Cyclin D1 in OE-STK38 transfected MDA-MB-231 and MDA-MB-468 cells. (H) Transwell assay in OE-STK38 transfected MDA-MB-231 and MDA-MB-468 to test the migration and invasion ability of cancer cells. All data were representative of at least three biological replicates and shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598