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Fig. 4 | Molecular Cancer

Fig. 4

From: A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer

Fig. 4

Biological functions of CAPG-171aa in TNBC. (A) qPCR of circCAPG and cell proliferation in OE-circCAPG and OE-circCAPG-ATG-mut transfected MDA-MB-231 and MDA-MB-468. (B) Ki67 immunofluorescence in OE-circCAPG and OE-circCAPG-ATG-mut transfected MDA-MB-231 and MDA-MB-468 (scale bars, 50 μm). (C) Colony formation capacity of OE-circCAPG and OE-circCAPG-ATG-mut transfected MDA-MB-231 and MDA-MB-468 (scale bars, 1 cm). (D) Cell cycle assay of OE-circCAPG and OE-circCAPG-ATG-mut transfected MDA-MB-231 and MDA-MB-468 based on flow cytometry. (E) IB of cell cycle biomarkers, cyclin D1 and CDK4 in OE-circCAPG and OE-circCAPG-ATG-mut transfected MDA-MB-231 and MDA-MB-468. (F) Transwell assay of OE-circCAPG and OE-circCAPG-ATG-mut transfected MDA-MB-231 and MDA-MB-468 (scale bars, 50 μm). (G) Wound healing assay of OE-circCAPG and OE-circCAPG-ATG-mut transfected MDA-MB-231 and MDA-MB-468 (scale bars,50 μm). All data were representative of at least three biological replicates and shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598