Fig. 2

Biological functions of circCAPG in TNBC. (A) qPCR of circCAPG and linear CAPG in circCAPG knockdown (KD) MDA-MB-231 and MDA-MB-468. The cell proliferation was estimated by OD450 value of circCAPG KD MDA-MB-231 and MDA-MB-468. (B) Colony formation assay of circCAPG KD in MDA-MB-231 and MDA-MB-468 (scale bars, 1 cm). (C) Representative immunofluorescence images of circCAPG KD MDA-MB-231 and MDA-MB-468 stained with Ki67 antibody (scale bars, 50 μm). (D) Immunoblot (IB) of Cyclin D1 and CDK4 in circCAPG KD MDA-MB-231 and MDA-MB-468. Anti-TUBULIN was used as a sample loading control. (E) Transwell assay in circCAPG KD MDA-MB-231 and MDA-MB-468 to estimate cell migration and invasion capacity (scale bars,50 μm). (F) BALB/c nude mice (n = 5 for each group) were injected with sh-circCAPG-1 or sh-scramble MDA-MB-231 cells (scale bars, 1 cm). The weights of xenograft tumors were summarized after animals were sacrificed. (G) Image of TNBC patient-derived organoids infected with lentivirus encoding control or circCAPG shRNAs. All data were representative of at least three biological replicates and shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001