Fig. 1

Construction of a global single-cell landscape of primary and metastatic LUAD. A Multiplex immunofluorescence map demonstrating CD34 expression in primary and metastatic LUAD tissues. Bar, 50 μm. B Flow chart of this study. The global single-cell landscape of primary and metastatic LUAD was constructed based on single-cell technology. C The single-cell atlas mapping cell types. D Bubble map showing cell marker genes for cell annotations. E Heat map showing copy number variation results to further validate the accuracy of cell annotation in the presence of multi-copy or low-copy number events in LUAD cells. F The difference in cell abundance between control, primary and metastatic LUAD