Fig. 8

Translational research of nanoinhibitor in organoid. A Western blot analysis of heat shock protein 90 level in organoids from a CRC tumor patient after treatment with PBS, EGCG 37 °C (50 mg/L), Nano 37 °C (50 mg/L), PBS 43 °C, EGCG 43 °C (50 mg/L), Nano 43 °C (50 mg/L). B Cell viabilities of organoid after treatment with EGCG 37 °C, Nano 37 °C, EGCG 43 °C and Nano 43 °C at the drug concentrations of 3.12, 6.25, 12.50, 25.00, 50.00, and 100.00 mg/L. C Microscopical images of organoids after treatment with different concentrations of nanoinhibitor. D AM/PI staining images of organoids after treatment with different concentrations of the nanoinhibitor. CC, Colorectal cancer. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. NS, not significant