Fig. 3
Antiproliferation mechanism of nanoinhibitor. A Brightfield images of tumor cells after treatment with the non-lethal high temperature (39 °C) once, twice, or thrice, respectively. Each heat session lasted for 30 min. B Cell viabilities after treatment with non-lethal high temperature (39 °C) corresponding to (A). C Western blot analysis of the effect of high temperature on the expression of heat shock proteins. D Docking models of EGCG bound to human HSP90α, and the structural view of EGCG in the HSP90 C-terminal domain. E Western blot analysis of HSP90 level in tumor cells treated with EGCG 37 °C (50 mg/L), nanoinhibitor 37 °C (50 mg/L), EGCG 43 °C (50 mg/L), or nanoinhibitor 43 °C (50 mg/L) at different time points. HSP901, HSP906, and HSP90.24, stand for HSP90 expression for 1, 6, 24 h after treatment, respectively. F Heat map of differentially expressed genes related to endoplasmic reticulum stress. G, H GSEA plots of differentially expressed genes enriched in (G) the protein processing in endoplasmic reticulum, and (H) oxidative phosphorylation. I ROS production after different treatments in CT26 tumor cells. *p < 0.05, **p < 0.01, and ****p < 0.0001