Fig. 5

Hsa_circ_0003258 promotes PCa metastasis through the hsa_circ_0003258/ miR-653-5p/Arhgap5 pathway. A QRT-PCR assay explored the overexpression and knockdown efficiency of miR-653-5p. B The qRT-PCR showing the ARHGAP5 mRNA level of the predicated targets with miR-653-5p mimic or miR-653-5p inhibitor in PCa cells. C Western blot showing the protein levels of ARHGAP5 after overexpression or silencing of miR-653-5p. D-E Cell migration observed in transwell assay after overexpression or silencing of miR-653-5p. F-G Transwell assay and Western blot was performed after transfection with indicated vectors, lv- hsa_circ_0003258, mimic-nc or mimic-miR-653-5p. H-I Transwell assay and Western blot detected the migratory capacity and the protein level of PCa cells after silencing ARHGAP5. ns: Not Significant; * P < 0.05; ** P < 0.01; *** P < 0.001; ****P < 0.0001