Fig. 3

miR-485-5p inhibits tumor progression. a Correlation analysis between PKMYT1AR and miR-485-5p using TCGA-LAUD dataset. b The decreased expression of miR-485-5p in TCGA-LAUD dataset. c The prognostic value of miR-485-5p in TCGA-LAUD examined by Kaplan-Meier Plotter. d The expression of miR-485-5p in fresh peripheral blood serum samples isolated from normal and NSCLC patients, respectively, verified by Real-time RT-PCR. e The expression of PKMYT1AR after over-expression of miR-485-5p, miR-216a-5p and miR-6884-5p in A549 cells examined by Real-time RT-PCR. f A schematic picture of the wild-type (WT) and mutant (MUT) PKMYT1AR luciferase reporter plasmids. g The luciferase activities of the PKMYT1AR luciferase reporters (WT or MUT) were examined in HEK-293T cells with miR-485-5p mimics or mimic NC co-expression. h miR-485-5p regulated A549 cell proliferation assay. i Relative miR-485-5p expression examined by Real-time RT-PCR assay in indicated cells. j-k miR-485-5p regulated A549 colony formation assay (j), (k) is the quantification data for (j). l-m miR-485-5p regulated A549 cell migration examined by wound healing (l) and trans-well (m) assays. Quantification data were also presented, and the OD₅₇₀ values for trans-well assay were indicated below. Scale bar=50 μm. n-p Representative xenograft tumor images (n), tumor masses (o) and tumor volumes (p) were shown for indicated groups. A549 cells were used. q-r Representative IHC staining of Ki67 (q) and CC3 (r) for indicated xenograft tumors. Quantification data were also indicated. Scale bar=50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001. mimics=miR-485-5p mimics, miR-NC=NC=miRNA mimics control, Anti-Ctrl=miRNA inhibitor control, WT=PKMYT1AR wild-type luciferase reporter, MUT=PKMYT1AR mutant luciferase reporter