Fig. 2

Validation of DHA-induced microRNA expression changes in exosomes secreted from breast cancer cells. MCF10A, MCF7, ZR751, MDA-MB-231 and BT20 breast cancer cells were plated at a density of 5x10⁶ cells per 150 mm dish and cultured for 3 days. Cells were treated with 100 μM DHA for 24 h. Exosomes were collected from the conditioned media by ultracentrifugcation, total RNA was extracted, reverse transcribed and amplified by real-time PCR using microRNA specific primers (n = 3, error bars = SE). The fold change in expression levels was calculated using the ΔΔCt method. *p < 0.05 by Student’s t test