Figure 3

Lack of stromal MMP13 leads to decreased tumor cell extravasation from the microvasculature. (a) Number of tumor cells in the liver at 24 and 48 hours post intrasplenic injection of MC38 tumor cells quantified as average number of cell tracker red labeled cells per 10X field from 5 random images per mouse (n = 3). (b) Quantification of the percentage of tumor cells extravasating in the liver at 24 and 48 hours post injection of wildtype and MMP13−/− mice (n = 3). Statistical analysis was performed using the 2-tailed t-test. (c) Representative image of 3D reconstruction of wildtype and MMP13−/− hepatic vasculature and (d) quantification of hepatic vascular volume in wildtype and MMP13−/− mice (n = 5). Statistical analysis was performed using the 2-tailed t-test.