Figure 5From: LMP1-augmented kappa intron enhancer activity contributes to upregulation expression of Ig kappa light chain via NF-kappaB and AP-1 pathways in nasopharyngeal carcinoma cellsCo-IP of endogenous p65 and p52. Non-denatured nuclear extracts prepared from HNE2-LMP1 cells were immunoprecipitated with anti-p65, anti-p52 or control IgG antibody. The immune complexes and the input (10% of the nuclear extracts used in the immunoprecipitation step) were analyzed by immunoblotting with antibody specific to p52 (upper panel, lanes 1-3) or p65 (lower panel, lanes 4-6). The same membrane was stripped and reprobed to detect p65 (lower panel, lanes 1-3) or p52 (upper panel, lanes 4-6).Back to article page