Figure 5

IAP knockdown inhibits proliferation. A. Cells were transfected with siRNA targeting cIAP-1 (0.5 nM), cIAP-2 (0.5 nM), XIAP (0.5 nM), or combinations thereof (as indicated in insert), and corresponding non-targeting control siRNA (siCon) to a final concentration of 1.5 nM for each group. Cells were grown in 6-well plates from 2 – 6 days, and stained with crystal violet as described in methods. Cell number is expressed as percent of control at time 0 days. Results are a representative of three independent experiments. B. Cells were transfected with the combination of all three siRNAs targeting cIAP-1 (0.5 nM), cIAP-2 (0.5 nM), and XIAP (0.5 nM) (siTarget), or corresponding non-targeting control siRNA (0.5 nM of each control siRNA to a final concentration of 1.5 nM) (siCon). Total cellular protein was extracted at days 2, 4 and 6 and western blot analysis of IAP knockdown was undertaken. Western blots are a representative of three individual experiments.