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Figure 2 | Molecular Cancer

Figure 2

From: The protein tyrosine phosphatase receptor type R gene is an early and frequent target of silencing in human colorectal tumorigenesis

Figure 2

Methylation analysis of PTPRR-1. (A): Schematic depiction of the PTPRR-1 CpG island encompassing the transcription start site (black arrow). CpG dinucleotides are represented as short vertical lines. Regions explored with combined bisulfite restriction analysis (COBRA) and with bisulfite genomic sequencing (BGS) on DNA extracted from fresh or formalin-fixed, paraffin-embedded (FFPE) samples are also shown. (B) and (C): Examples of COBRA analysis and BGS in cell lines (B) and clinical samples (C). COBRA: Arrows indicate BstUI-digested DNA fragments representing methylated alleles; slower-migrating fragments correspond to undigested, unmethylated DNA. BGS: Each row shows the methylation status of a cloned target sequence, with circles representing unmethylated (open) and methylated (filled) CpG dinucleotides.

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Molecular Cancer

ISSN: 1476-4598