Table 1 SAGE tags significantly increased in metastatic melanoma cells (MMC) compared to normal melanocytes (NM)
GATC preceded Tag | Count | p value | GenBank match [accession number] | |
|---|---|---|---|---|
| Â | NM | MMC | Â | Â |
Translation, ribosomal structure and biogenesis | ||||
ACATCCATCA | 28 | 73 | 0 | Ss 40S ribosomal protein S20, RPS20 [AY550070] |
AAACCAAAGA | 1 | 10 | 0.004 | Ss cDNA [AJ681350]homolog to Hs small nuclear ribo- nucleoprotein polypeptides B and B1, SNRPB [BC080516] |
TGACTATAAC | 18 | 35 | 0.007 | Ss cDNA [BX923125] homolog to Hs ribosomal protein L24, RPL24 [BC000690] |
AAGTTCCCGC | 17 | 32 | 0.012 | Ss cDNA [BX674755] homolog to Hs ribosomal protein L18a, RPL18A [BC066319] |
AACCTAATTA | 58 | 77 | 0.025 | Ss 40S ribosomal protein S12, RPS12 [NM_214363] |
AACTCAATAA | 44 | 62 | 0.027 | Ss ribosomal protein L10a, RPL10A [NM_001097477] |
AAAGATTAAG | 20 | 32 | 0.040 | Ss ribosomal protein L27, RPL27 [NM_001097479] |
Signal transduction mechanisms | ||||
ATTGTAGATG | 13 | 31 | 0.002 | Ss guanine nucleotide beta like protein GNB2L1, RACK1 [NM_214332] |
AGTTATGAAG | 0 | 5 | 0.028 | Ss cDNA [CN160727] homolog to Hs ras-related GTP-binding protein, Rheb [D78132] |
AAGCTACACA | 4 | 11 | 0.043 | Ss cDNA [CJ016486] homolog to Hs calmodulin 1 [phosphorylase kinase, delta], CALM1 [BC011834] |
Transcription | ||||
CAGAGGGACA | 1 | 10 | 0.004 | Ss cDNA [CN 157150] homolog to Hs retinoblastoma binding protein, RbAp48 [X74262] |
ACAACTGGGG | 0 | 6 | 0.014 | Ss cDNA [CJ020293] homolog to Hs general transcription factor IIB, GTF2B [NM_001514] |
TGATAGAAGA | 6 | 15 | 0.025 | Ss cDNA [CF792678] homolog to Hs activating transcription factor 4, ATF4 [BC073754] |
TATGAATAAG | 4 | 11 | 0.043 | Ss non-metastatic cells 1 protein NM23A [NM_001044610] |
Secondary metabolites biosynthesis, transport and catabolism | ||||
AGTATCAACA | 24 | 46 | 0.002 | Ss TYRP1 tyrosinase related protein1 [AB207240] |
Secretion | ||||
TGCTCAGGCT | 0 | 13 | 0.000 | Ss cDNA [BX674961] homolog to secernin |
Energy production and conversion | ||||
AATACAAGTT | 3 | 12 | 0.014 | Ss NADH dehydrogenase [ubiquinone]1 alpha subcomplex 4 NDUFA4 [NM_001097468] |
Posttranslational modification, protein turnover | ||||
GAGGTGGAGA | 3 | 10 | 0.043 | Ss cDNA [CJ014518] homolog to Hs peptidylprolyl isomerase B [cyclophilin B], PPIB [NM_000942] |
General prediction only | ||||
ATTTCTAGGC | 0 | 5 | 0.028 | Ss cDNA [CK455473] homolog to Hs CDC10 cell division cycle 10 homolog [S. cerevisiae], CDC10 [NM_001788] |
Function unknown | ||||
TCACCCGCAA | 0 | 9 | 0.0015 | No reliable matches |
TCGTCCCTGT | 0 | 8 | 0.0031 | Ss cDNA [BX665592] |
CCTGTGCTGA | 0 | 7 | 0.0064 | Ss cDNA [AJ647874] |
GGTCATTCAT | 0 | 7 | 0.0064 | No reliable matches |
TCGCCTGGAC | 0 | 7 | 0.0064 | No reliable matches |
TCGTCCCTTC | 0 | 7 | 0.0064 | No reliable matches |
ATTCATGTCA | 3 | 13 | 0.0079 | Ss cDNA [BP164587] |
GTCTAATCAC | 2 | 10 | 0.0134 | No reliable matches |
AATGACCGAC | 0 | 6 | 0.0147 | No reliable matches |
CACCCGCAAT | 0 | 6 | 0.0149 | No reliable matches |
CCTTCCGACT | 0 | 6 | 0.0149 | No reliable matches |
CGTCCCTGTG | 0 | 6 | 0.0149 | No reliable matches |
AGATAATTTG | 0 | 5 | 0.0285 | Ss cDNA clone [EW659942] |
ATAGACGAGC | 0 | 5 | 0.0285 | No reliable matches |
CTGCATTGCT | 1 | 7 | 0.0321 | Ss cDNA [BX922537] |
AGAATATAAG | 5 | 13 | 0.0341 | No reliable matches |
CCGCGTTGCT | 41 | 57 | 0.0362 | Ss cDNA [AJ666089] |
ATGAAGATAT | 2 | 8 | 0.0434 | Ss cDNA [CB286104] |
TGCTGCAGGG | 4 | 11 | 0.0435 | Ss cDNA [CA780804] |