Figure 5

Gβγ may transmit the signal from Smo to Gli via JNK. Values of the blots (p-JNK) relative to JNK quantified by Image J are statistical different (data not shown). A-B, K562/A02 (A) and KB/VCR cells (B) were treated with PTX (100 ng/ml) or transfected with Gαt plasmids, and cells were harvested for western blot analysis. K562 and KB cells were used as negative control. Blots are representative results from at least three separate experiments. C, K562/A02 cells were pre-treated with PTX (100 ng/ml) for 1 h followed by exposure to SAG (100 nM) for 10 mins. Blots are representative results from at least three experiments. D, K562/A02 cells were transfected with GFP or Gαt plasmids, and were stimulated with SAG (100 nM) for 10 mins. The immunoblots are representative of three separate experiments with identical results. E, NIH-3 T3 cells transfected with Gli luciferase reporter and pRL-Renilla luciferase plasmids plus GFP or Gβ1γ2 plasmids were treated with JIP for 36 h, and were collected for dual luciferase reporter assay. The results are represented as fold induction relative to GFP cohort, and were represented as mean ± s.d. from three separate experiments.