Figure 1

Influence of Aurora-A overexpression on cell cycle. (A) Wi-38 cells were infected with adenoviruses expressing either Aurora-A or control (lacZ) proteins. Overexpressed protein levels were compared to T98G (glioblastoma), VM24, and VM7 (melanoma) cell lines. (B) Wi-38 cells overexpressing Aurora-A were tested in a growth curve experiment against cells infected with a control virus. The results of two independent experiments are depicted. (C) Logarithmically growing Wi-38 cells were infected with the viruses expressing the indicated proteins and DNA content was measured 24, 48 and 72 hours after infection. The time curve shows the percentage of cells in G1 and S-phase from a representative experiment. The histograms and the respective cell cycle analysis of the 72 hours time point is depicted. Bars illustrate means and standard deviation of four independent experiments. Statistical analysis was done using an unpaired, two-sided t-test. * P < 0.05.