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Fig. 5 | Molecular Cancer

Fig. 5

From: Phosphoproteomic analysis reveals Smarcb1 dependent EGFR signaling in Malignant Rhabdoid tumor cells

Fig. 5

Smarcb1 dependent EGFR phosphorylation and transcriptional de-regulation. a Western blot demonstrating constitutive phosphorylation of Tyr-1092 of the EGFR in Smarcb1 deficient cells, and down-regulation of the total-EGFR protein. b Smarcb1 dependent transcriptional regulation of the ErbB family receptors and the EGF ligand. Graph shows RNA levels relative to beta actin as estimated by qRT-PCR. Note that the Y axis of the right panel is two orders of magnitude lower, indicating that expression levels of ErbB3, 4 and EGF are significantly low. In both cell lines Egfr and ErbB3 showed reduced expression in Smarcb1 proficient cells. * Fold change pMIG/Smarcb1 167 = 2.4, 365 = 4.5; T-test P.V; 167 = 0.00058, 365 = 0.0098. ** Fold change pMIG/Smarcb1 167 = 8.2, 365 = 6.8; T-test P.V; 167 = 0.0078, 365 = 0.013

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