Fig. 3

Differential morphology of Smarcb1 proficient and deficient cell lines. a Whilst in Smarcb1 proficient cell lines actin forms stress fibers across the cell, in Smarcb1 deficient cell lines the actin is found diffused throughout the cytoplasm. In red – Phalloidin, In blue – Dapi b Reduced adhesiveness of Smarcb1 deficient cell lines as evident in an adhesion assay. Cells were allowed to adhere for 20/40 min before quantification of adherent fraction was carried out as described in the materials and methods section. T-test; 167 20', P.V = 0.0015; 167 40', P.V = 0.0027; 365 20', P.V = 0.0008; 365 40', P.V = 0.0011. c Differential morphology, size and number of focal adhesion sites as visualized via Paxillin staining. In red – Paxillin, In blue – DAPI. d Western blot showing a slight increase in PAX following Smarcb1 expression, consistent with available expression data