Table 6 Details of the primers used for the amplification of IL-1, IFN-γ, COX-2, IL-8, c-Myc, and Bcl-2 with the corresponding targets and PCR conditions.

β-actin for COX-2, IL-1β, IL-8, and Bcl-2

F-5'-GATGAGATTGGCATGGCTTT-3'

R-5'-CACCTTCACCGTTCCAGTTT-3'

15 s 95°C, 20 s 60°C, 60 s 72°C

Maximbio, USA

[48]

COX-2

F-5'-TCCTATTATACTAGAGCCCTTCCT-3'

R-5'-TTCCACAATCTCATTTGAATCAGG-3'.

15 s 95°C, 20 s 60°C, 30 s 72°C

Maximbio, USA

[48]

IL-1β

F, 5'-TCCAGGGACAGGATATGGAG-3',

R, 5'-TCTTTCAACACGCAGGACAG-3' (133 bp);

20 s 95°C, 60 s 60°C, 30 s 72 s

Promega,

[49]

IL-8

F-TAGCAAAATTGAGGCCAAGG

R-GGACTTGTGGATCCTGGCTA

20 s 95°C, 60 s 60°C, 30 s 72 s

DNASTAR, USA

[50]

Bcl-2

F-5'acatcgccctgtggatgact3'

R-5'gggccgtacagttccacaaa3

95°C 3 min, 40 cycles 95°C 30 s, 58°C 30 s, and 73°C 30 s

Promega, USA

[51]

β-actin for IFN-γ and c-Myc

F-5'-GATGGCCACGGCTGCTT-3'

R-5'-ACCCTCATTGCCAATGGT-3'

20 s 95°C, annealing/extension 60 s 60°C

Maximbio, USA

[52]

IFN-γ

F-5'-CAGCTCTGCATCGTTTTGGG-3'

R-5'-GTTCCATTATCCGCTACATCTGAA-3'

20 s 95°C, annealing/extension 60 s 60°C

DNASTAR, USA

[52]

c-Myc

F-TCAAGAGGTGCCACGTCTCC

R-TCTTGGCAGCAGGATAGTCCTT

30 s 95°C, 70 s 60°C (annealing/extension).

Maximbio, USA

[50]