Table 2 PCR and DNA sequencing primers

RB1M

5'-GGG AGT TTC GC G GAC GTG AC-3'

5'-ACG TCG AAA CAC G CC CCG-3'

172

65.0

RB1U

5'-GGG AGT TTT GT G GAT GTG AT-3'

5'-ACA TCA AAA CAC A CC CCA-3'

172

61.0

RB1 promoter

5'-CGC CCC AGT TCC CCA CAG A-3'

5'-GGC AAC TGA GCG CCG CGT-3'

163

53.0

RB1 exon 1

5'-AAC GGG AGT CGG GAG AG-3'

5'-AAT CCT GTC ACC ATT CTG C-3'

412

55.2

RB1 exon 2

5'-GAT TAT TTT CAT TTG GTA GGC-3'

5'-AAA GTG GTA GGA TTA CAG GC-3'

351

51.3

RB1 exon 3

5'-TTT TAA CAT AGT ATC CAG TGT GTG-3'

5'-TAC ACT TTC ATA ACG GCT CC-3'

350

54.4

RB1 exon 4

5'-GAC CCT TCG TTT TCT TAT ATT CTC-3'

5'-ATC AGA GTG TAA CCC TAA TAA AAT G-3'

390

55.2

RB1 exon 5

5'-ATT GGG AAA ATC TAC TTG AAC-3'

5'-TCA AAC TAA CCC TAA CTA TCA AG-3'

265

54.2

RB1 exon 6

5'-CAT TCT ATT ATG CAT TTA ACT AAG G-3'

5'-CTA ACA GTT AAT AAG CCA AGC AG-3'

340

53.6

RB1 exon 7

5'-ATG GAT ATA CTC TAC CCT GCG-3'

5'-ATC CTG TCA GCC TTA GAA CC-3'

291

55.2

RB1 exon 8

5'-TAA AAG TAG TAG AAT GTT ACC AAG-3'

5'-CAG TGA TTC CAG AGT GAG G-3'

470

55.2

RB1 exon 9

5'-TTG ACA CCT CTA ACT TAC CCT G-3'

5'-TTG GCT AGA TTC TTC TTG GG-3'

301

55.7

RB1 exon 10

5'-GAA ATC TGT GCC TCT GTG TG-3'

5'-AAA GGT AAC TGT TAT AGG ACA CAC-3'

200

52.9

RB1 exon 11

5'-GTT ATC AAT ACC ACC AGG GAG-3'

5'-CAA ATC TGA AAC ACT ATA AAG CC-3'

443

53.0

RB1 exon 19

5'-AGA CAA GAT GTA TCT GGG TGT AC-3'

5'-CAT GAT TTG AAC CCA GTC AG-3'

306

53.6

RB1 exon 20

5'-CTT ATT CCC ACA GTG TAT GCC-3'

5'-AGC CTG GGT AAC AGA GTG AG-3'

341

47.5

RB1 exon 21

5'-ATT CTG ACT ACT TTT ACA TC-3'

5'-TTA TGT TAT GGA TAT GGA T-3'

192

58.0

RB1 exon 22.1

5'-ATA TGT GCT TCT TAC CAG T-3'

5'-CAC GTT TGA ATG TCT GAG GA-3'

148

53.5

RB1 exon 22.2

5'-CCT CAG ACA TTC AAA CGT GT-3'

5'-TTG GTG GAC CCA TTA CAT TA-3'

175

54.1

RB1 exon 23

5'-TAA TGT AAT GGG TCC ACC AA-3'

5'-TCA AAA TAA TCC CCC TCT CA-3'

277

55.6

RB1 exon 24

5'-GAA TGA TGT ATT TAT GCT CA-3'

5'-TTC TTT TAT ACT TAC AAT GC-3'

165

46.1

RB1 exon 25

5'-CTT TGC CTG ATT TTT GAC AC-3'

5'-CAG TGC TGA GAC TCT GGA TTC-3'

270

56.3

RB1 exon 26

5'-CAT TTA TGT TTT AGA TGG TTA G-3'

5'-GTT TAT TTC GTT TAC ACA AG-3'

318

46.8

RB1 exon 27

5'-CAG CCA CTT GCC AAC TTA C-3'

5'-CAT AAA CAG AAC CTG GGA AAG-3'

230

53.5

RB1-1.r-S2/AS4

5'-AAC GGG AGT CGG GAG AG-3'

5'-GAA TTA CAT TCA CCT CTT CAT CAA G-3'

1204

45.0

RB1-1.r-S3/AS2

5'-ATG ATA AAA CTC TTC AGA CTG ATT C-3'

5'-TGT CCA CCA AGG TCC TGA G-3'

1794

45.0

RB1-2.r-frag1-S/AS

5'-AGG AGG ACC CAG AGC AGG AC-3'

5'-CCA AGA AAC TTT TAG CAC CAA TG-3'

496

45.0

RB1-2.r-frag2-S/AS

5'-CTA CTG AAA TAA ATT CTG CAT TGG T-3'

5'-CTC TTC ATC AAG GTT ACT TTT TCG T-3'

528

45.0

RB1-2.r-frag3-S/AS

5'-GAA ACA CAG AGA ACA CCA C-3'

5'-ATT CTG AGA TGT ACT TCT GCT A-3'

461

45.0

RB1-2.r-frag4-S/AS

5'-AGC AAA CTT CTG AAT GAC AAC-3'

5'-GAG AGG TAG ATT TCA ATG G-3'

518

45.0

RB1-2.r-frag5-S/AS

5'-CTC CAA AGA AAA AAG GTT CAA-3'

5'-GGT ATT GGT GAC AAG GTA GG-3'

512

45.0

RB1-2.r-frag6-S/AS

5'-GTA TTC TAT AAC TCG GTC TTC A-3'

5'-CAT TTC TCT TCC TTG TTT GA-3'

526

45.0

RB1-plasmid-S1/AS1

5'-GGT TTT TCT CAG GGG ACG-3'

5'-GTG AGA GAC AAT GAA TCC AGA G-3'

45.0

RB1-plasmid-S/AS-STOP

5'-CAC AGC TCG CTG GCT CCC-3'

5'-TTT CTC TTC CTT GTT TGA G-3'

45.0

RB1 (Leu607Ile)

5'-GCA GCA GAT ATG TAT ATT TCT CCT GTA AGA TCT CC-3'

RB1 (Arg621Cys)

5'-AAA GGT TCA ACT ACG TGT GTA AAT TCT ACT GC-3'

RB1 (Arg698Trp)

5'-GAA CTC ATG AGA GAC TGG CAT TTG GAC CAA ATT ATG-3'

D13S153 F/R

5'-TTG CAC TGT GGA GAT AAA CAC ATA G-3'

5'-TCA CAT TGT CTT TTA AGG CAG GAG-3'

D13S263 F/R

5'-CCT GGC CTG TTA GTT TTT ATT GTT A-3'

5'-CCC AGT CTT GGG TAT GTT TTT A-3'

RB1

5'-TGT ATC GGC TAG CCT ATC TC-3'

5'-AAT TAA CAA GGT GTG GTG G-3'