Figure 5
miR-21 expression in carcinomas and normal prostate tissues. (A) Quantification of miR-21 expression levels (top panel) in carcinomas and matched normal tissues obtained from 36 patients subjected to radical prostatectomy. Data are reported as RQ of miR-21 expression with respect to an internal calibrator (RWPE-1). Analysis of miR-21 expression (bottom panel) reported as Log10 of the ratio tumor/matched normal tissue for each patient (RQtumor/RQnormal). (B) miR-21 expression levels (average RQ ± SD) as a function of nodal status, extraprostatic extension (EPE) of the disease and Gleason score. (C) Representative RT-PCR and western immunoblotting showing the expression of miR-21 validated target genes in untreated and LNAScr- or LNA21-treated DU145 cells, at days 2 and 3 after transfection. (D) Scatterplot showing the lack of a correlation between RQ expression values of miR-21 and PTEN mRNA in clinical specimens.