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Figure 4 | Molecular Cancer

Figure 4

From: miR-21: an oncomir on strike in prostate cancer

Figure 4

Analysis of miR-21 knockdown on the radiosensitivity profiles of PCa cells. (A) Clonogenic survival curves of untreated (black triangle), LNAScr (black circle)- or LNA21 (white circle)-transfected cells calculated on day 12 after exposure to increasing doses (2-8 Gy) of γ- radiation. Data shown on the plots represent the inter-experiment averages (± SD) calculated from at least three intra-experiment averages. (B) Representative immunofluorescence analysis of γ- H2AX induction in LNAScr- or LNA21-treated DU145 cells exposed to γ- radiation (4 Gy). Nuclei were counterstained with 4',6-diamidino-2-phenylindole. Scale bar: 10 μm. Magnification: × 40. NR, no radiation. (C) Quantification of γ- H2AX foci in DU145 (top panel) and PC-3 cells (bottom panel). Data are reported as percentage of γ- H2AX-positive cells in the overall cell population (mean values ± SD).

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