Tissue Inhibitor of Metalloproteinases-4. The road less traveled

Table 2 Inhibitory activity of TIMP-4 on matrix metalloproteinases.

TIMP-4	Target MMP	Kinetic parameter	Key Residue(s)	Ref.
full-length	cdMMP-1¹	K_i2.59 nM	?	[57]
full-lenght	cdMMP-1¹	IC₅₀ 19 nM	?	[55]
full-lenght	MMP-1	K_i0.65 nM	?	[57]
N-TIMP-4²	MMP-2	K_i^app 0.3 nM	?	[33]
full-lenght	MMP-2	K_i^app ≤ 9 pM	?	[35]
full-lenght	MMP-2	IC₅₀ 3 nM	?	[55]
full-lenght	cdMMP-3¹	K_i1.24 nM	?	[57]
full-lenght	MMP-3	IC₅₀ 45 nM	?	[55]
full-lenght	MMP-3	K_i1.51 nM	?	[57]
full-lenght	MMP-7	IC₅₀ 8 nM	?	[55]
N-TIMP-4	cdMMP-8¹	K_i9.7 nM	Ser2	[32]
N-TIMP-4	cdMMP-9¹	K_i14.5 nM	Ser2	[32]
full-lenght	MMP-9	IC₅₀ 83 nM	?	[55]
full-lenght	MMP-26	IC₅₀ 0.4 nM	?	[56]
N-TIMP-4	mbMT1-MMP³	K_i^app 1.0 nM	?	[33]
full-lenght	sMT1-MMP³	K_i^app 0.1 nM	?	[35]
N-TIMP-4	ADAM-17/TACE	Ki 8.68 nM	?	[65]
full-lenght	ADAM-17/TACE	Ki 180 nM	?	[65]
full-lenght	cdADAM33¹	Ki 220 nM	?	[62]

The table shows the kinetic parameter of the TIMP-4 target matrix metalloproteinase (MMP) and, when available, the key residue involved in the interaction.
¹ Catalytic domain of the metalloproteinase.
² Amino-terminal domain of TIMP-4.
³ mbMT1-MMP and sMT1-MMP refer to membrane-bond and soluble forms of Membrane Type 1 Matrix Metalloproteinase, respectively.
K_i= Inhibitory constant; IC₅₀ = Median inhibitory constant; K_i^app = Apparent inhibitory constant. ? = not studied.

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