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Figure 4 | Molecular Cancer

Figure 4

From: The novel chemokine receptor CXCR7 regulates trans-endothelial migration of cancer cells

Figure 4

Agents that block CXCL12 binding to CXCR7 inhibit CXCL12-potentiated NC-37 cell TEM to CXCL13 and CCL19. A-B: NC-37 cell TEM to 300 nM CXCL13 (A) or 1 μM CCL19 (B) in the presence of 10 nM CXCL12 in both wells was performed in the additional presence of the indicated compounds (red bars), chemokines (blue bars), or mAbs (green bars). Controls (black bars) include TEM in the absence of chemokines, presence of CXCL13 or CCL19 only, presence of CXCL12 only, and presence of CXCL13 or CCL19 and CXCL12 with no other additions. C-D: NC-37 cells were analyzed in bare filter chemotaxis assays to 100 nM CXCL13 (C) or 5 nM CCL19 (D) in the presence of the indicated compounds (red bars), chemokines (blue bars), or mAbs (green bars). Controls (black bars) include chemotaxis in the absence or presence of chemokine with no other additions. * p-value < 0.05 by Student's t-test comparing the indicated treatment vs. matched control. For A and B, the mean ± SEM of triplicate wells per treatment is shown; for C and D, the mean ± SEM of six wells per treatment is shown. Representative of 3 independent experiments.

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